anti α 1a  (Proteintech)

Journal: Neuron

Article Title: Radial astrocyte synchronization modulates the visual system during behavioral-state transitions

doi: 10.1016/j.neuron.2023.09.022

Figure Lengend Snippet:

Article Snippet: We used the primary antibody rabbit anti-α-1a AR (Cat. #PA1-047, Invitrogen™, Waltham, MA, USA), diluted in 1:100.

Techniques: Recombinant, Software, Imaging, Optogenetics

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Hey2 enhancer activity defines unipotent progenitors for left ventricular cardiomyocytes in juxta-cardiac field of early mouse embryo.

doi: 10.1073/pnas.2307658120

Figure Lengend Snippet: Fig. 1. Transcriptional activity of the Hey2 enhancer during early cardiac development. (A) β-galactosidase activity in Hey2 enhancer-LacZ transgenic (Tg) embryos was evaluated from the gastrulation to heart tube stages. Arrows indicate the enhancer-active regions. HT, heart tube; SHF, second heart field; LV and RV, left and right ventricles; OFT, outflow tract; LB, late bud stage; EHF, early head-fold stage; LHF, late head-fold stage; ss, somite stage. (Scale bars, 200 μm.) (B and C) LacZ transcript and β-galactosidase expression in Hey2 enhancer-LacZ Tg embryos at early and late phases of cardiac crescent formation (early and late CC) was compared with the expression of myocardial markers Tnnt2 and myosin heavy chain (MyHC) using RNAscope analysis and immunohistochemistry, respectively. LacZ-expressing cells emerged prior to the expression of myocardial markers at the early CC phase, and undifferentiated LacZ-expressing cells were present rostral to the differentiating myocardium at the late CC phase (arrows). (Scale bars in B and C, 100 μm.)

Article Snippet: The following primary antibodies were diluted 1/500 in the blocking solution and incubated with embryos or sections for 16 to 20 h at 4 °C: α- β- galactosidase (rabbit, kindly provided by Nicolas JF, Pasteur Institute), α- CD31 (rat, BD #550274), α- estrogen receptor (ESR) (rabbit, Abcam #ab16660), α- GFP (rabbit, MBL #598), α- GFP (rat, Nacalai #04404- 26), α- myosin heavy chain (mouse, DSHB #MF20), α- RFP (rabbit, MBL #PM005), and α- WT1 (rabbit, Abcam #89901).

Techniques: Activity Assay, Transgenic Assay, Expressing, RNAscope, Immunohistochemistry

Journal: PLoS ONE

Article Title: 2D and 3D-Organized Cardiac Cells Shows Differences in Cellular Morphology, Adhesion Junctions, Presence of Myofibrils and Protein Expression

doi: 10.1371/journal.pone.0038147

Figure Lengend Snippet: 24-hs cardiac cell cultures were incubated with BrdU (3 ug/mL) and stained with anti-BrdU antibody. 2D-isolated cells were analyzed by phase contrast ( a–b , gray) and conventional fluorescence microscopy ( a–b , red), while 3D-aggregates were analyzed by confocal microscopy ( c–g ). Images ( a , b ) are different magnifications. BrdU-positive nuclei are found in both 2D and 3D-cells, but are more frequent in 2D-cells ( a–g ). Selected confocal slices, 10 µm apart, from bottom to top are shown in images ( c–e ). Note the difference in the distribution of BrdU positive cells along the aggregate Z-axis ( c–e ). All focal planes were merged in image ( f ), where it is possible to see the whole aggregate because of the faint fluorescence background. The relative position of all cells can be better visualized in the depth-color coded image shown in ( g ). The colored scale represents slice count, from bottom to top, each 2 µm apart. Scale bars in images ( a, b, c, f ) correspond to 50 µm.

Article Snippet: Mouse monoclonal anti-BrdU, mouse monoclonal anti-α-tubulin (clone DM 1A), mouse monoclonal anti-sarcomeric alpha-actinin (clone EA53), rabbit polyclonal anti-desmin, rabbit polyclonal anti-connexin 43, and rabbit polyclonal anti-pan-cadherin antibodies were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

Techniques: Incubation, Staining, Isolation, Fluorescence, Microscopy, Confocal Microscopy